www.alphalabs.co.uk 3 Vanadate Oxidation Method Wako’s method for bilirubin determination was developed to address some of the common interferences and is unaffected by haemoglobin up to 500 mg/dL. It delivers reliable results from haemolysed specimens, negating the need to reject samples. Introducing Wako’s direct bilirubin assay could help improve your laboratory service and facilitate patient management, by helping to avoid diagnostic delays and unnecessary repeat sample collection. Since traditional diazo methods are often subject to interference from haemoglobin, they create a risk of reporting values below the true concentrations. Therefore laboratories may find themselves rejecting a significant number of samples which exhibit haemolysis. This problem is most commonly seen in paediatric samples and can impact the care of this vulnerable patient group. Rejection of haemolysed samples necessitates additional specimen collection from the patient. The Wako Direct Bilirubin assay is not significantly affected by haemolysis up to an index of 800: levels that are commonly seen in neonatal samples. The use of this assay prevents rejection of samples as unsuitable for analysis and has been adopted by many laboratories. Within the Wako Assays Bilirubin is oxidised to biliverdin and the change in absorbance as measured in the yellow part of the spectrum is used to calculate the bilirubin content of the sample. Both Direct and Total Bilirubin assays provide excellent performance with minimal interference, delivering clear, reliable answers when it matters. References: www.nice.org.uk/guidance/cg98 www.old.bspghan.org.uk/sites/default/files/ guidelines/2016_guideline_for_the_investigation_ of_neonatal_conjugated_jaundice.pdf Hb (mg/dL) Hb (mg/dL) For more information, visit our website alphalabs.co.uk/bilirubin Direct and Total Bilirubin Use Assays with Confidence No interference from Ascorbic Acid up to 50mg/dL No interference from Haemoglobin up to 500mg/dL Direct Bilirubin: linear to 20mg/dL (342µmol/L) Total Bilirubin: linear up to 40mg/dL (684µmol/L) Ready-to-use liquid stable reagents Open-vial stability 30 days at 2-8C Standardised to SRM916a (NIST)
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