Leading Edge 2021 Issue 2

Find out more at: www.alphalabs.co.uk/fpela 3 New Method: SGH Comp. site: York Allowable di erence ±10% Passing-Bablok t (y=-1.293 + 0.9756 x) 500 450 400 350 300 250 200 150 100 50 0 0 50 100 150 200 250 300 350 400 450 500 Figure 1. Comparison of results of faecal samples tested with the fPELA assay at both York Hospital and Scunthorpe General Hospital. Like many hospitals we are tight on space, so there is no capacity for additional equipment which means we needed to investigate what was possible with our existing platforms – the BÜHLMANN fPELA was the obvious place to start as there was a protocol available for the Roche c702. Setting the assay up on the analyser was reasonably straight forward. The only limitation is that as a third-party assay we can only have one set of reagents loaded per assay. So, we are double filling the reagent packs which reduces the number of times that we need to replace the reagent. This approach also helps with the amount of shots per kit, because you reduce the dead volume impact. We already use the CALEX extraction devices for our calprotectin samples. This was an advantage as the team was already familiar with processing them. We have found that around 10% of samples request both calprotectin and elastase, so we only need to do one extraction for both assays. Previously we requested that separate samples were provided for elastase and calprotectin due to the different stability recommendations of each assay. It is possible that moving forward more samples will require both assays performed, because they can come as a single request. During the evaluation we did a sample swap with PathLinks, Scunthorpe General Hospital that was also evaluating the fPELA, and the results were within 10% of each other, so we were satisfied with that. [Figure 1] With the new service we are preparing the samples daily, storing them in the fridge and then running the assay twice a week – this will give a much better turnaround time on the results for our clinicians. We have been live with the assay for a few months now, and the service is running very smoothly. ■BÜHLMANN fPELA® turbo Runs on main stream clinical chemistry analysers for a simplified workflow and rapid results ■CALEX® sample extraction Utilises the same sample preparation device as the fCAL turbo calprotectin assay. ■Revolutionise your elastase workflow Perform two tests from a single extraction ■Time to first result: 10 minutes ■Dynamic range: 10 - 5000µg/g ■Extraction: CALEX ■Kit size: ~100 tests New BÜHLMANN fPELA® turbo Faecal Pancreatic Elastase Assay We would certainly recommend the BÜHLMANN fPELA to other hospitals considering the assay – the workflow and direct reporting has made things so much easier, and it would certainly be good to have other users to compare results to on the EQA.”