14 Liquid Handling n web: www.alphalabs.co.uk Nanoject Accessories Two lengths of borosilicate glass (N-51-A) capillaries are available for use with Nanoject II –3.5” and 7” and are ready for pulling to the required tolerance. Once the tips are pulled, they must be “backfilled” with oil (or other non- compressible fluid) before attachment to the injector. Silicone or mineral oil is frequently used for this purpose. Backfilling is performed using a 30 gauge x 2” needle and a syringe. The precise operation of the Nanoject Auto Injectors depends greatly on the use of tips prepared from the specific glass provided. For this reason, it is recommended that only Drummond microcapillaries are used. Micromanipulators The injector head should be mounted on a micromanipulator or stereotaxic unit using the Universal adapter supplied in the kit. The Marzhauser MM33 micromanipulator is a popular, manual model equipped with high precision and stable cross-roller bearings, which work constantly without any noticeable wear even after many years of intensive use. The MM33 also has a number of ergonomic benefits. The control knobs for all three axes are arranged at the same level so that it can be easily operated without looking. In addition, the operational controls are at the back of the unit, allowing several devices to be easily installed side by side. A support base is available for the MM3 micromanipulator and is also compatible with other brands. For convenient hands-free operation, an optional foot switch can be used to control the Nanoject II and Nanoject III remotely. 3-000-024-R 3-000-025-SB Injector Tips/Replacement Glass Capillaries Catalogue Number O.D (mm) Length I.D (mm) Pack Size 3-000-203-G-X 1.14 3.5” 0.53 100 3-000-203-G-XL 1.14 7” 0.53 100 Footswitch for NanoJect III Injector Tips Nanoject Accessories Nanoject II Replacement Parts Catalogue Number Description 3-000-024-A Universal Adaptor for Nanoject II* 3-000-030-A Replacement Injector for Drummond Nanoject II™ 3-000-027 Backfilling Needle for Nanoject II Auto Injector 3-000-000-203-X Wire Plunger for Nanoject II Auto Injector * Can be used on stereotaxic equipment using 7.9mm clamps Nanoject Accessories Catalogue Number Description 3-000-024-R Micromanipulator Model MM33 Right-Handed Configuration 3-000-024-L Micromanipulator Model MM33 Left-Handed Configuration 3-000-025-SB Support Base for Micromanipulator Replacement Parts
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