Laboratory Equipment - Electrophoresis 10 n web: www.alphalabs.co.uk Electrophoresis - DNA-View DNA-View Electrophoresis System Traditional electrophoresis methods use a combination of harmful UV light and toxic ethidium bromide to visualise DNA samples in a gel. Intense UV light such as that typically used in transilluminators, can cause skin burns and damage to eyes in a very short exposure time. In addition UV can cause cross-linking of DNA and mutagenesis, thereby reducing cloning efficiency. Ethidium Bromide, though still one of the most widely used DNA stains for electrophoresis, is a known carcinogen and mutagen and should always be used with caution. Blue LED lights can also be used to cause emission of fluorescence although the wavelength is different to UV, and fluorescence is never as strong as more traditional UV and stain combinations. But with safe proprietary stains, and blue LED illumination, it is possible to visualise DNA gels, without the worry of handling harmful chemicals, or exposure to UV light. The DNA-View system enables you to do this. What’s more, the DNA-View system enables you to see your samples separating in real time, and it can even be used to simplify sample recovery by using a 2 tier Well approach. Built around the versatile Choice Horizontal Gel system, DNA-View comprises a bluView base station which incorporates the blue LED illuminator and power supply. The base station exactly fits the Choice Gel tank which is supplied with a special lid incorporating a light filter and extractor fan which eliminates condensation to optimise visualisation and resolution during electrophoresis. For completeness, a selection of combs are included suitable for gel resolution or sample preparation. Features n Versatile, highly compact, self-contained system: for convenience n Flexible high resolution system: for many different applications n Integrated tank, power supply and gel illuminator: saves time and space n Safety for users: no dangerous UV n Safety for samples: no mutagenesis of DNA, no compromise on cloning efficiency n Real time visualisation of gel run: no over-runs n Simplifies DNA elution: simple ethanol precipitation from buffer n No need for expensive pre-made gels: lab-cast gels work perfectly and save you money n Current Choice gel tank users upgrade available: cost-effective solution n Optional Gel Documentation System with 12.1 mega- pixel digital camera n Complete ready-to-use system excluding chemicals and reagents Applications n Resolution of DNA samples n Simple DNA recovery n Track DNA without harmful UV n Save on time-consuming gel elution techniques n Teaching electrophoresis A Safer Alternative to Traditional Electrophoresis DNA-VIEW Catalogue Number Description EL1560 DNA-View system complete with 15 x 7cm gel tray, 1 set of casting dams EL1565 DNA-View system complete with 15 x 10cm gel tray, 1 set of casting dams EL1570 DNA-View system complete with 15 x 15cm gel tray, 1 set of casting dams EL1575 DNA-View system complete with 15 x 7, 15 x 10 and 15 x 15cm gel trays, 3 sets of casting dams EL1555 DNA-View base station & bluVIEW lid EL1580 DNA-View gel documentation hood with 12.1MP camera All DNA-View systems are supplied with 8 double-sided and 4 extra thick preparatory combs
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