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Microbiology 320 QuickFISH™ Rapid Molecular Diagnostic Tests Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk AdvanDx QuickFISH™ Rapid Molecular Diagnostic Tests Sepsis Diagnostics - The Problem Sepsis is a systemic inflammatory response to a microbial infection (including bloodstream infections such as bacteraemia and fungaemia) which can lead to organ dysfunction. It is a serious and potentially fatal condition, making prompt diagnosis and treatment vital. When a blood culture is flagged as positive a gram stain is taken to identify the organism responsible for the infection. While this is helpful, tailored therapeutic intervention relies on identification to species level, which can take a further 24-48 hours using conventional techniques. This can result in inappropriate therapies being used, or treatment proceeding using broad spectrum antibiotics until definitive species identification can be obtained. A number of approaches have been tried to speed up species diagnosis. Biochemical assays (such as the coagulase assay used to characterise Staphylococci) are widely used but are not always completely accurate and may not provide a result for several hours. Molecular assays based on RT-PCR or nanoparticles have also come onto the market, but have a limited species range and require expensive equipment. One of the most promising new technologies is MALDI-TOF. This technique can theoretically identify any organism and has been adopted by a number of clinical microbiology laboratories. While it has proven to be effective in many cases, it has also been seen to have limitations. These include identification of organisms in the case of polymicrobial bacteraemia, and the identification of organisms with more robust cell walls such as yeasts and some gram-positive bacteria. In addition the presence of charcoal or resin in the culture may prove problematic for MALDI-TOF. Sepsis Diagnostics - A Solution Published data demonstrates that the risk of death from septic shock increases by the hour unless appropriate therapeutic intervention occurs1. The QuickFISH rapid molecular diagnostic system overcomes these issues by enabling a positive species identification to be obtained from a positive blood culture in just 20 minutes. This allows the pathogen identity to be reported to the clinician at the same time as the gram stain results- 2 days earlier than using conventional methods. Providing this information permits appropriate antimicrobial therapy to commence promptly, potentially resulting in enhanced patient outcomes. In addition the ability to identify possible misleading contaminants, such as coagulase negative Staphylococci (CNS), allows treatment to be promptly de- escalated where necessary, with attendant resource savings. ■■Optimise patient care ■■Reduce mortality rates ■■Shorten length of hospital stay ■■Lower health care costs The QuickFISH system is based on the patented PNA-FISH technology that enhances the well-known technique of Fluorescent In Situ Hybridisation (FISH) by incorporating the use of Peptide Nucleic Acids (PNA). QuickFISH is a rapid, simple slide based assay requiring only 5 minutes hands-on time. It provides a definitive pathogen identification, directly from a positive blood culture, in just 20 minutes. It is ideally suited to providing accurate diagnosis of bacteraemia, candidaemia and sepsis in the clinical microbiology lab. QuickFISH compares favourably with biochemical tests, amplification tests and MALDI-TOF. Considering commercially available molecular tests, QuickFISH has the fastest turn around time.2 References 1. Kumar et al. Chest. 2009 Nov;136(5):1237-48). 2. Deck, M.K. et al. Rapid detection of Enterococcus spp. Direct from blood cultures using Enterococcus QuickFISH method: a multicentre investigation. Diagn Microbiol Infect Dis 2013, Article in Press DOI: 10.1016/j.diagmicro-bio. 2013.12.004


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