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Faecal Testing Calprotectin ELISA: Quantitative High Throughput Test Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 266 How Does It Work? The BÜHLMANN Calprotectin ELISA kit is designed for the quantitative determination of human calprotectin concentrations in stool samples. After a short extraction procedure, the test allows for the selective antibody measurement of calprotectin antigen by sandwich ELISA. The capture monoclonal antibody coated on the microtitre plate is highly specific for calprotectin heterodimeric and polymeric complexes (4-5) respectively. Clinical Evidence A study at the University Hospital Basel, Switzerland, proves calprotectin concentrations measured with the BÜHLMANN Calprotectin ELISA correlate highly with clinical results and the current gold standard of colonoscopy. 405 symptomatic patients were included. With a sensitivity of 84% and a specificity of 95% the BÜHLMANN calprotectin ELISA confirmed the nature of the pathology behind the symptoms, and showed an excellent negative predictive value of 93%. In this study, the performance of the BÜHLMANN monoclonal ELISA was superior to polyclonal calprotectin determinations (Figure1) and to lactoferrin measurements (not shown). The cut off used was 50 μg/g calprotectin in faeces. The BÜHLMANN Calprotectin ELISA (EK-CAL) is the UK market-leading calprotectin ELISA. It provides quantitative determination of faecal calprotectin in high sample throughput environments. The format is a microtitre plate based assay that can be performed manually or on an automated ELISA system to reduce hands-on time. The BÜHLMANN ELISA also has an extended range format for use in monitoring patient therapy and mucosal healing. This reduces the need for further dilution and repeated assay. Why Use EK-CAL Calprotectin ELISA? n A simple and well documented assay n Rapid ELISA system (total ELISA time is 75 minutes) n All reagents are ready to use (except wash buffer) n The kit includes all calibrators and quality controls ready to use n Measurable range is 10-600 μg/g or 30-1800 μg/g: same test kit, different protocol n Reliable results over the entire range including the higher concentrations (compared to other monoclonal ELISA’s) n Easily automated on ELISA processors n CE marked protocol available for the DS2 and DSx Figure 1: ROC analysis of the ability of BÜHLMANN Calprotectin ELISA and of a polyclonal ELISA to discriminate between patients with CD and IBS. From Lehmann. et al. (in prep). Calprotectin ELISA Catalogue Number Description Pack Size EK-CAL Calprotectin ELISA 96 wells EK-CAL2 Calprotectin ELISA 192 wells BÜHLMANN Laboratories Calprotectin Assays


Catalogue_WEB_WoP_Jun16
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