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Catalogue_WEB_WoP_Jun16

Clinical Chemistry Vitamin B6 Enzymatic Assay The Vitamin B6 enzymatic assay is intended for the quantitative determination of Pyridoxal 5’-phosphate (PLP, Vitamin B6) in EDTA plasma. Measurement of Vitamin B6 allows for detection of potential deficiency or overdose. n Dual reconstituted/liquid stable reagent Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 248 n Assay range: 9-250nmol/L n Open-vial stability: Substrate, Dilution Buffer, Enzyme Buffer, Enzyme - 60 days at 2-8C Apo-enzyme, Calibrators and Controls - 60 days at ≤ -20C n Calibrators and Controls included Technical information: L-Tyrosine is decarboxylated by a vitamin B6 (PLP)-depent enzyme, tyrosine-apo- decarboxylase to tyramine. The activity of the apo-enzyme is directly proportional to the amount of PLP present in the reaction mixture. Tyramine is then oxidised to p-hydroxybenzyl aldehyde and hydrogen peroxide (H2O2) by the action of tyramine oxidase. The H2O2 reacts with 4-aminoantipyrine and n-ethyl-n-(2-hydroxy-3- sulfopropyl)-m-toluidine (TOOS) in the presence of horseradish peroxidase to obtain a quinoneimine (purple dye) whose absorbance is measured at 546nm (520-595nm). Vitamin B6 Enzymatic Assay Catalogue Number Description Pack Size KK-VB6 Vitamin B6 Enzymatic Assay 100 tests BÜHLMANN laboratories Total 25-OH-Vitamin D Competitive ELISA for quantification of 25-OH-Vitamin D (Vitamin D2 and Vitamin D3) in human serum or plasma. Vitamin D is a steroid hormone involved in the intestinal absorption of calcium and the regulation of calcium homeostasis. In the liver, Vitamin D is hydroxylated to give 25-Hydroxyvitamin D (25-OH D), the major circulating metabolite of Vitamin D. Both Vitamin D and 25-OH D enter the circulation bound to Vitamin D binding protein (VDBP). Determination of 25-OH D in serum or plasma can assist diagnosis and therapeutic control of postmenopausal osteoporosis, rickets in children, osteomalacia, renal osteodystrophy, neonatal hypocalcemia and hyperparathyroidism. n Competition ELISA n Total 25-OH-Vitamin D (D2 and D3) n Dynamic range: 2.89-130ng/ml Technical information: Samples are pre-treated with denaturing buffer to extract the vitamin D from the VDBP complex. Once neutralised the samples are mixed with enzyme conjugate and enzyme complex. Endogenous 25-OH Vitamin D in the sample competes with the 25-OH Vitamin D3 biotin conjugate to bind to the VDBP coating the microtitre plate. Bound 25-OH Vitamin D-biotin is subsequently detected by peroxidase-labelled streptavidin. Colour intensity following addition of enzyme substrate is inversely proportional to the concentration of 25-OH Vitamin D in the sample. Total 25-OH-Vitamin D Catalogue Number Description Pack Size Z13001 Total 25-OH-Vitamin D ELISA 96 wells Dialab Clinical Chemistry - V


Catalogue_WEB_WoP_Jun16
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