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Pricing and Online Ordering at www.alphalabs.co.uk Clinical Chemistry Urea Enzymatic determination of urea in serum, plasma and urine. Urea is the end product of amino acid catabolism and is partly reabsorbed in the glomerular filtration; therefore it is an indicator of renal function. Blood urea levels can vary according to the protein content of diet, to hepatic function and to protein catabolism during tissue breakdown. n Dual liquid-stable reagent n Linearity: up to 300 mg/dL n Open-vial stability: 90 days when stored at 2-8C Technical information: In presence of glutamate dehydrogenase (GLDH), urea is hydrolysed by urease into carbon dioxide and ammonia. These react with ketoglutarate and ß-NADH2 forming ß-NAD in an equivalent amount. By measuring the variation in absorbance due to the oxidation of ß-NADH2 in a fixed time interval, it is possible to calculate the level of urea in the sample. Urea Catalogue Number Description Pack Size 17629H Urea Liquid Reagent 3x65ml,1x20ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Uric Acid Determination of uric acid with an enzymatic-colorimetric method in serum and urine. Uric acid and its salts are end products of purine metabolism. In gout, the most common complication of hyperuricemia, increased levels of uric acid lead to the formation of monosodium urate crystals in skeletal joints. Other hyperuricemia complications are caused by renal diseases with decreased excretion of waste products, drug abuse and increased alcohol consumption as well as the use of certain therapeutic agents. High uric acid levels also constitute an indirect risk factor for coronary heart disease. Hypouricemia is seldom observed and associated with rare hereditary metabolic disorders. n Single liquid-stable reagent n Measuring range: Serum: 0.3-2.5 mg/dL Urine: 0.02-1.49 mmol/L n On-board stability is typically 60 days Technical information: Uric acid is transformed by uricase into allantoin and hydrogen peroxide. The latter, in conjunction with the catalytic influence of peroxidase, oxidises the (4-aminoantipyrine/TOOS*) chromogen to form a coloured compound, the intensity of which is proportional to the amount of uric acid present in the sample. * = N-ethyl-N-(2-hydroxy)-3-methylanilin propan-sulphonate sodic al Uric Acid Catalogue Number Description Pack Size 17658H Uric Acid Liquid Colorimetric Assay 4x65ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Triglycerides Enzymatic-colorimetric determination of triglycerides in serum and plasma. Triglycerides are esters of glycerol with three fatty acid groups. Measurement of triglycerides indicates lipid status and detects risk of atherosclerosis. High triglyceride levels also occur in various diseases of liver, kidneys and pancreas. n Single liquid-stable reagent n Linearity: up to 1000 mg/dL n On-board stability is typically 60 days Technical information: Triglycerides are hydrolysed by lipoproteinlipase (LPL) to glycerol and fatty acids. Glycerol is then phosphorylised, in the presence of glycerolkinase (GK) and adenosine 5-triphosphate (ATP), into glycerol-3-phosphate. Further conversion of this molecule by glycerol-3-phosphate oxidase (GPO) yields dihydroxyacetonephosphate and hydrogen peroxide. In the presence of peroxidase (POD), the hydrogen peroxide oxidises the chromogen 4-aminoantipyrine and TOOS* to form a red compound, the colour intensity of which is proportional to the concentration of triglycerides in the sample. * = N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline Triglycerides Catalogue Number Description Pack Size 17624 Triglycerides Liquid Colorimetric Assay 6x65ml 34Q021 Lipids Control 3x2ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel UIBC Determination of UIBC (unsaturated iron-binding capacity) in serum. Transferrin is a transport protein in blood, carrying iron. The additional amount of iron that can be bound is the unsaturated iron-binding capacity (UIBC). The sum of serum iron and UIBC represents the total ironbinding capacity (TIBC). UIBC measurements are used in the diagnosis and treatment of anaemia. n Dual reconstituted/liquid-stable reagent n Linearity: up to 500 μg/dL n Reconstituted reagent stability: 60 days when stored at 2-8C n Includes standard Technical information: Serum is added to an alkaline buffer/reducing agent solution containing a known concentration of iron to saturate the available binding sites on the transferrin. The iron that remains free after transferrin saturation is reduced to ferrous state and then complexed by ferene-S to form a stable coloured complex. UIBC is determined by subtracting the quantity of unbound iron from the total added quantity. UIBC Catalogue Number Description Pack Size 17639 UIBC Liquid Reagent 2x40;1x11ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Clinical Chemistry - T/U n email order sales@alphalabs.co.uk n freefax order 0800 614249 247


Catalogue_WEB_WoP_Jun16
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