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Clinical Chemistry Non-Esterified Fatty Acids (NEFA) For the determination of non-esterified Fatty Acids in serum by an enzymatic colorimetric method (ACS-ACOD-MEHA). Non-esterified fatty acid (NEFA) in serum binding albumin is used as an important energy source for peripheral tissues. The amount of NEFA in serum depends on a balance between intake in liver and peripheral tissues, and the release from adipose tissues. The amount of NEFA decreases by physical exercise and increases by starvation, cold, fear or smoking. An increase or decrease of NEFA is observed in diabetes, hepatic diseases or endocrine diseases. Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 244 n Dual reconstituted reagent system n Linearity: Up to 4.00 mEq/L n Reconstituted reagent stability: 10 days when stored at 2-10C Technical information: Non-esterified fatty acid (NEFA) in sample is converted to Acyl-CoA, AMP and pyrophosphoric acid (PPi) by the action of Acyl-CoA synthetase (ACS), in the presence of coenzyme A (CoA) and adenosine 5-triphosphate disodium salt (ATP). The resulting Acyl-CoA is oxidised and yields 2,3-trans-Enoyl-CoA and hydrogen peroxide by the action of Acyl-CoA oxidase (ACOD). In the presence of peroxidase (POD), the hydrogen peroxide formed yields a blue purple pigment by quantitative oxidation condensation with 3-Methyl-N-Ethyl-N-(‚-Hydroxyethyl)- Aniline (MEHA) and 4-aminoantipyrine (4-AA). Non-esterified fatty acids (NEFA) concentration is obtained by measuring absorbance of the blue/purple colour. NEFA Catalogue Number Description Pack Size 434-91795 NEFA-HR(2) R1 Assay 4x50ml 436-91995 NEFA-HR(2) R2 Assay 4x25ml 270-77000 NEFA Standard 2x10ml 410-00104 Control Serum I (Normal) 4x5ml 416-00202 Control Serum II (Abnormal) 10x5ml Wako Chemicals Neutrophil-Gelatinase Associated Lipocalin (NGAL) ELISA Assay NGAL is a novel biomarker for diagnosing Acute Kidney Injury (AKI). The key advantage of NGAL is that it responds earlier than other renal status markers like serum Creatinine and shows a proportionate response to injury. NGAL determination thus permits the early diagnosis and prognostic stratification of AKI. n 12x8 coated microwells and frame n Measured range: Urine 4.3 - 12.0 ng/mL Plasma 4.2 - 10.5ng/mL n Open-vial stability: Diluted wash solution concentrate - 4 weeks at 2-8C Diluted sample diluent concentrate - 24 hours at 2-8C Reagents, Calibrators and Controls - until expiry date at 2-8C Technical information: The assay is an ELISA performed in microwells coated with a monoclonal antibody against human NGAL. Bound NGAL is detected with a horseradish peroxidase conjugated monoclonal antibody and the assay is developed by incubation with a colour-forming substrate. The assay is a rapid 2-step procedure. NGAL ELISA Assay Catalogue Number Description Pack Size KIT 037 NGAL Rapid ELISA Kit 96 wells BioPorto Phospholipids Direct enzymatic-colorimetric determination of phospholipids in serum and plasma. The determination of phospholipids in serum is important in the diagnosis of liver diseases. High levels of serum phospholipids can be found in Niemann-Pick disease and in the cholesterol lecithin acyltransferase deficiency. n Single reconstituted reagent n Linearity: up to 1000 mg/dL n Reconstituted reagent stability: 14 days when stored at 2-8C Technical information: Phospholipids are hydrolysed, in presence of phospholipase D, to choline and phosphatidic acid. The choline is then oxidised by choline oxidase into betaine and hydrogen peroxide, which, in the presence of peroxidase, reacts with 4-aminoantipyrine and a phenol-derivative to form a red compound. The colour intensity of this compound is proportional to the amount of phospholipids contained in the sample. Phospholipids Catalogue Number Description Pack Size 17320H Phospholipids Colorimetric Assay 5x10ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Clinical Chemistry - N/P


Catalogue_WEB_WoP_Jun16
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