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Clinical Chemistry Lipase Kinetic colorimetric direct determination of lipase in serum and plasma. Lipase enzymes are produced in the pancreas and are also secreted in small amounts by the salivary glands and the gastric, pulmonary and intestinal mucosa. Determination of lipase is used for the diagnosis and treatment of diseases of the pancreas (such as acute and chronic pancreatitis) and obstruction of the pancreatic duct. Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 242 n Dual liquid-stable reagents n Linearity: up to 250 U/L n On-board stability is typically 30 days Technical information: The method for the determination of lipase is based on the cleavage of a specific chromogenic lipase substrate 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6’-methylresorufin)-ester emulsified in stabilised micro-particles. In the presence of specific activators of pancreatic lipase, such as as colipase, calcium ions and bile acids, the substrate is converted into 1,2-O-dilauryl-racglycerol decomposes spontaneously into glutaric acid and methylresorufin. The increase of absorbance due to methylresorufin formation is proportional to the activity of lipase in the sample. Lipase Catalogue Number and glutaric acid-6’-methylresorufin-ester. The latter compound Description Pack Size 17616H Lipase Colorimetric Assay 4x50ml;2x21ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Lipoprotein (a) (Lp(a)) (Turbidimetric) For the determination of Lp(a) in serum or plasma. The structure of Lp(a) was found to be quite similar to that of plasminogen. In consideration of the structural characteristics of Lp(a) it was proposed that Lp(a) was related to atherosclerosis. Cholesterol is one of the diagnostic aids for the diagnosis of atherosclerosis. Lp(a) is considered another risk factor, which is independent of cholesterol. n Dual liquid-stable reagent n Linearity: 0-100 mg/dL n Open-vial stability: until expiry date when stored at 2-8C Lipoprotein (a) (Lp(a)) Catalogue Number Description Pack Size 418-41801 Lp(a)-HA Reagent 1 (Buffer) 4x30ml 414-41901 Lp(a)-HA Reagent 2 (Antibody) 2x10ml 417-41491 Lp(a) Calibrator 2x1ml 410-00104 Control Serum I (Normal) 4x5ml 416-00202 Control Serum II (Abnormal) 10x5ml Wako Chemicals Lactate Dry-Fast Determination of lactate with an enzymatic-colorimetric method in serum, plasma and cerebrospinal fluid (CSF). Lactate is produced in the Cori cycle, by anaerobic conversion of glucose, mainly in skeletal muscle. Lactate determination, frequently in conjunction with pyruvate, is used in the diagnosis of lactic acidosis due to reduced tissue oxygenation or enzymatic deficiencies. n Linearity: up to 150 mg/dL n Reconstituted reagent stability: 30 days when stored at 2-8C n Includes standard Technical information: Lactate is oxidised by lactate oxidase to pyruvate and hydrogen peroxide which, in the presence of peroxidase (POD), reacts with TOOS* to form a compound, the colour intensity of which is proportional to the concentration of lactate in the sample. * = N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline Lactate Dry-Fast Catalogue Number Description Pack Size 17285 Lactate Dry-Fast Colorimetric Assay 5x10ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Lactate Dehydrogenase (LDH) Kinetic determination of LDH according to SFBC mod. recommendations in serum or plasma. LDH is an enzyme that can be found in the cytoplasm of all tissues with the highest concentrations in liver, heart and skeletal muscle. Increased LDH activities are found in cardiac muscle diseases or lung and kidney tumours. Determination of LDH is useful for the diagnosis and monitoring of some liver diseases. It is recommended to perform other organ-specific diagnostic tests to confirm pathology. n Dual liquid-stable reagents n Linearity: up to 1200 U/L n On-board stability is typically 30 days Technical information: LDH catalyses the reaction between pyruvate and ß-NADH2 with formation of lactate and ß-NAD. By measuring the variation of absorbance due to the oxidation of ß-NADH2 in a fixed interval of time, it is possible to calculate the LDH activity in the sample. Lactate Dehydrogenase (LDH) Catalogue Number Description Pack Size 17294H LDH Liquid Kinetic Assay 3x65ml;1x19ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Clinical Chemistry - L


Catalogue_WEB_WoP_Jun16
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