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Pricing and Online Ordering at www.alphalabs.co.uk Clinical Chemistry Creatine Kinase - NAC (CK-NAC) Kinetic determination of CK activity according to IFCC recommendations in serum and plasma. CK catalyses the reversible phosphorylation of creatine by ATP. CK is a dimer composed of two subunits which form three active isoenzymes: BB (CK-1), MB (CK-2), MM (CK-3). Elevated CK values are due to muscular damage and associated pathologies. CK determination, usually performed with CK2 (also called CK-MB), is used for the diagnosis and follow-up of AMI (acute myocardial infarction) and some muscular diseases. n Dual liquid-stable reagent n Linearity: up to 1000 U/L n On-board stability is typically 30 days Technical information: CK catalyses the reaction between creatine phosphate and ADP forming creatine and ATP. The ATP generated, in presence of glucose and hexokinase (HK), gives ADP and glucose-6-phosphate. The glucose-6-phophate formed, in the presence of glucose-6-phosphate dehydrogenase (G6P-DH), reacts with β-NADP+ forming 6-phosphogluconate and β-NADPH. The increase in absorbance due to the conversion of β-NADP+ to β-NADPH is proportional to the activity of CK. Creatine Kinase - NAC (CK-NAC) Catalogue Number Description Pack Size 17296H CK-NAC Liquid Kinetic Assay 1x65ml;1x18ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Creatinine (Enzymatic) Enzymatic, colorimetric method for determination of creatinine in serum, plasma and urine. Renal function is monitored using estimated glomerular filtration rate based on the measurement of creatinine. n Ready to use reagents. n On-board stability is typically 30 days n Analytical range 0.02-50.0mg/dL Technical information: This enzymatic colorimetric method is based on creatinine metabolism. Creatinine in the sample is enzymatically converted to creatine which is subsequently broken down to give sarcosine and urea. The resulting sarcosine is broken down further by sarcosine oxidase to produce hydrogen peroxidise which reacts with the reagent to form a chromogen. The colour intensity is proportional to the creatinine in the sample. Creatinine (Enzymatic) Catalogue Number Description Pack Size 17654H Creatinine Enzymatic Assay 2x50ml;2x18ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Creatinine (Jaffé Method) Kinetic, modified Jaffé method for quantitative determination of creatinine in serum, plasma or urine. In healthy individuals with normal renal function creatinine is eliminated by glomerular filtration rate. As such, creatinine is used to monitor renal disease and kidney function n Ready to use reagents n On-board stability is typically 30 days n Analytical range 0.05-15.0 mg/dL Technical information: In alkaline solution, creatinine will form a coloured complex with picric acid. The colour intensity as measured at a fixed time interval is proportional to the concentration of creatinine in the sample. Creatinine (Jaffé Method) Catalogue Number Description Pack Size 17609H Creatinine Assay 3x65ml;3x65ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel Cystatin (Turbidimetric) For the quantitative immunoturbidimetric determination of cystatin C in serum and plasma. Cystatin C is a non-glycosylated basic protein that is produced at a constant rate in most nucleated cells and is freely filtered through normal Glomerular membranes. It is then re-absorbed and almost entirely catabolised in the proximal tubules. Therefore, cystatin C concentration in human blood is proportional to the glomerular filtration rate (GFR). A reduction in GFR causes a rise in the concentration of cystatin C. Cystatin C concentration is not significantly influenced by other factors such as muscular mass, inflammatory diseases, sex, age or diet. n Dual liquid-stable reagent n Measuring range: 0.4-8.0 mg/L n Open-vial stability: 9 weeks when stored at 2-8C n Includes calibrators and controls Technical information: The determination is based on a turbidimetric reaction between a specific antiserum and cystatin C. The change in absorbance caused by the immuno-complex is proportional to the concentration of the analyte in the sample. Cystatin (Turbidimetric) Catalogue Number Description Pack Size KK-CYC Cystatin C Turbidimetric Assay 1x55;1x10ml KK-CYC-6* Cystatin C Turbidimetric Assay 1x55;1x10ml *contains pre-diluted calibrators for use on Beckman/Olympus (AU) and Ortho (Vitros 5.1) analysers. BÜHLMANN Laboratories Clinical Chemistry - C n email order sales@alphalabs.co.uk n freefax order 0800 614249 235


Catalogue_WEB_WoP_Jun16
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