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Clinical Chemistry Cholesterol - Low-Density Lipoprotein (LDL) For the determination of LDL cholesterol in serum or plasma. A strong positive relationship has been reported between LDL cholesterol concentrations and the incidence of CHD. LDL cholesterol is used as a predictive test for patients at risk of developing CHD. Cholinesterase Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 232 n Dual Liquid Reagent System n Linearity: Up to 400 mg/dL n Open-vial stability: 4 weeks when stored at 2-10C Technical information: When a sample is mixed with Reagent 1, the protecting reagent binds to LDL and protects LDL from enzyme reactions. Cholesterol esterase (CHE) and cholesterol oxidase (CO) react with non-LDL lipoproteins (chlyomicrons (CM), very low density lipoproteins (VLDL) and HDL). Hydrogen peroxide produced by the enzyme reactions with non-LDL cholesterol is decomposed by catalase in Reagent 1. When Reagent 2 is added, the protecting reagent is removed from LDL and catalase is inactivated by sodium azide. In this second process, CHE and CO reacts only with LDL-C. Hydrogen peroxide produced by the enzyme reactions with LDL-C yields a colour complex. By measuring the absorbance of the blue colour complex produced, at approximately 600nm, the LDL-C concentration can be calculated. Cholesterol - LDL Catalogue Number Description Pack Size 419-24017 LDL-Cholesterol R1 Kit 4x60ml 419-24027 LDL-Cholesterol R2 Kit 4x20ml 419-24032 LDL-Cholesterol Calibrator 4x1ml Wako Chemicals Kinetic colorimetric determination of cholinesterase activity according to DGKC recommendations in serum and plasma. Cholinesterase (ACHE), also known as Acetylcholine Acetylhydrolase EC 3.1.1.7, is found in erythrocytes, in lung and spleen and in the grey matter of brain. Pseudocholinesterase (CHE), also referred to as Acylcholine Acylhydrolase EC 3.1.1.8, is found in serum, liver, pancreas, heart and the white matter of brain. Decreases in serum cholinesterase (CHE) activity are useful to diagnose liver disorders, hepatitis, cirrhosis, carcinum with metastasis, sensitivity to succinylcholine administration and pesticide poisoning. n Dual liquid-stable reagent n Linearity: up to 22,000 U/L n On-board stability is typically 30 days Technical information: Cholinesterase catalyses the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. Thiocholine reduces hexacyanoferrate (III) to hexacyanoferrate (II). The decrease in absorbance is directly proportional to CHE activity in the sample. Cholinesterase Catalogue Number Description Pack Size 17019H Cholinesterase Liquid Colorimetric Assay 2x50;1x20ml 16550 Clin Chem Calibrator 4x3ml 16150 Clin Chem Control 1 6x5ml 16250 Clin Chem Control 2 6x5ml Sentinel CH50 - Total Complement Activity For the determination of total complement activity in serum. Complement activity is a direct indicator of abnormalities of the complement system. It has been correlated with the active stage of systemic lupus erythematosus (SLE), rheumatoid arthritis and inherited deficiencies of the complement system. n Dual reconstituted reagent n Linearity: up to 65 U/ml n Reconstituted reagent stability: 40 days when stored at 2-10C Technical information: The kit uses liposomes, which are vesicles made from cell membrane components. When a sample is mixed with the liposomes and the substrate, the antibodies in the reagent combine with dinitrophenyl (DNP) on the liposomes and then complement molecules in the sample are activated by the antigen-antibody complex. The activated complement breaks the membrane of the liposome. The enzyme, G6PDH, contained in the liposome, reacts with NAD and glucose 6-phosphate (G6P) in the reagent. During this reaction the NAD is reduced to NADH. The absorbance increase is proportional to the complement activity in the sample. CH50 - Total Complement Activity Catalogue Number Description Pack Size 995-40801 Autokit CH50 R1: 2x20ml, R2: 1x20ml 997-43801 CH50 Calibrator 5x0.5ml 991-43701 Complement Control 10x0.5ml Low/10x0.5ml High Wako Chemicals Clinical Chemistry - C


Catalogue_WEB_WoP_Jun16
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