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Clinical Chemistry Carbon Dioxide Quantitative colorimetric method for determination of CO2 in human serum or plasma. Metabolic activity in the body constantly produces carbon dioxide (CO2) which is transported by the blood to the lungs for eliminations. The majority of carbon dioxide present in serum is in the form of bicarbonate. n Single ready to use reagent n Linearity up to 50mmol/L n Sensitivity: 2.9mmol/L Cellular Allergen Stimulation Test (CAST) Systems In vitro allergy testing has become important in reducing the number of time consuming, costly and potentially harmful in vivo challenges to determine allergy specificity in patients. The new Flow2 CAST® system represents a significant step forward in in vitro allergy testing, allowing the simple detection of Basophil Activation on whole blood samples. The Flow2 CAST system, which replaces the Flow CAST system, uses a dual labelling system to identify basophils in whole blood samples, negating the requirement for lengthy and labour intensive leukocyte isolation steps. Using a flow cytometer, basophils are identified in the sample by using a specific marker (CCR3) and then the level of CD63 is analysed on this sub-population to give a read out of the response to a purified allergen which is added to the sample. In the CAST-2000® ELISA, leukocytes isolated from patient blood are simultaneously primed with Interleukin 3 (IL-3) and stimulated with allergens. The ELISA measures de novo synthesis of the allergic sulfidoleukotriene mediators (LTC4, LTD4 and LTE4) as a quantitative read-out of the patient’s response to the allergen. The Flow2 CAST system, together with the CAST 2000 ELISA, is particularly suited for the following types of allergies: n Drug hyper-sensitivites (e.g. anaesthetics, antibiotics, NSAIDs) n Food allergies n Bee and wasp stings The complete package for allergy diagnosis also includes a library of approximately 150 standardised allergens. Please enquire for a comprehensive list of available allergens. Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 230 Technical information: Phosphoenol pyruvate and bicarbonate ions are converted in the presence of magnesium by phosphoenol pyruvate carboxylase (PEP-C) to give oxaloacetate and dihydrogen phosphate. The oxaloacetate formed is subsequently converted to malate by malate dehydrogenase, an enzymatic decarboxylation process during which NADH is reduce to NAD+. The decrease in absorbance resulting from NADH oxidation is proportional to the original concentration of CO2 in the sample. Carbon Dioxide Catalogue Number Description Pack Size N00043/20 Carbon Dioxide PEP-C Colorimetric Assay 5x50ml D06520SV Carbon Dioxide Standard 1x3ml D98481SV Diacon Normal Control 1x5ml D98482SV Diacon Abnormal Control 1x5ml Dialab CAST Systems Catalogue Number Description Pack Size EK-CAST CAST2000 ELISA 192 wells FK-CCR Flow2-CAST - Basophil Activation Test 100 tests Various Purified Allergens 4 stimulations BÜHLMANN Laboratories Clinical Chemistry - CAST Allergen Assays


Catalogue_WEB_WoP_Jun16
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