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Pricing and Online Ordering at www.alphalabs.co.uk Research Reagents Electrophoresis Buffers and Reagents In electrophoresis a wide range of buffers and reagents are required in addition to the high quality gel media now available. These reagents must be of at least equal quality to the separation medium in use. Alpha Laboratories can offer a select range of convenience buffers. Without having to weigh, mix and adjust pH, convenience products avoid the simple errors, which can mean the loss of complete experiments due to the tedious nature of preparing routine solutions. The specifications offer a high degree of experimental security in laboratory work across a wide range of scientific disciplines. This means they satisfy the exacting demands of modern science and are ideal for use in electrophoresis. Purposefully developed and specified for these particular applications they guarantee the kind of results expected with high quality chemicals. All these features mean these products are essential for the modern laboratory. n Save time n Reduce opportunities for error n Allow high quality staff to be used more effectively n Guaranteed consistent reagents n All batches tested and validated prior to use n Reduce exposure to hazardous materials The products are supplied as concentrates for simple dilution. TAE/TBE Buffers These buffers are widely used in the preparation of nucleic acid electrophoresis gels. All convenience buffers are formulated with 18 Megohm Water and 0.2 Micron Filtration. TAE Buffer (50x) is a concentrated solution of 2M Tris-Acetate and 100mM Sodium EDTA in distilled/deionised water (pH 8.3 at 1x concentration). TBE Buffer is available as a 10x concentrated buffer solution of Tris-Borate-EDTA in distilled/deionised water, containing 0.089M Tris base, 0.089M boric acid (pH8.3) and 2mM Na2EDTA. TE Buffers TE Buffer is a 100x concentrated solution of 1M Tris-HCl, pH8, with 100mM EDTA. Manufactured from ultra pure reagents, this buffer is the standard for DNA and RNA purification, processing and storage. TE Buffer Catalogue Number Description Pack size EL0081 TE Buffer 100x concentrate 25ml Triple Loading Buffer Tracking dyes are routinely used in both protein and nucleic acid electrophoresis to monitor the progress of electrophoresis and indicate at what point the gel should be stopped. Generally they are added to the sample prior to loading on to the gel (loading buffer). Alpha Laboratories offers a convenient ready-made triple loading buffer with three tracking dyes (Bromophenol Blue, Xylene Cyanol, and Orange G). The Triple Dye Loading Buffer (6x) is a non-denaturing loading buffer for native polyacrylamide and agarose gel applications. In normal type agaroses in 0.5x TBE the dye mobility is broadly unaffected over the range of 0.5 to 1.4% w/v. The dyes run as follows (linear double stranded DNA); Orange G 50bp Bromophenol blue 300bp Xylene cyanol 4kbp Triple dye loading buffer contains 50% (w/v) sucrose and 40mM Tris base in distilled/deionised water. TAE/TBE Buffers Catalogue Number Description Pack size EL0077 TAE Buffer 50x conc. 1L EL0079 TBE Buffer 10x conc. 1L EL0080 TBE Buffer 10x conc. 4L Triple Loading Buffer Catalogue Number Description Pack size EL0084 Triple Loading Buffer 1.2ml Electrophoresis Reagents n email order sales@alphalabs.co.uk n freefax order 0800 614249 215


Catalogue_WEB_WoP_Jun16
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