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Research Reagents Agarose 1 x TAE buffer range (bp) Gel concentration (%) 1 x TBE buffer range (bp) Application Universal 20,000 - 1,000 0.6 15,000 - 500 Nucleic acid analytical and preparative 214 Electrophoresis Reagents Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk Separation Ranges for Different Buffers with Application Guide Agarose Specifications at a Glance Agarose Catalogue Number Description Pack size EL0014 Agarose Universal 100g EL0015 Agarose Universal 500g EL0018 Agarose Extra 100g EL0020 Agarose Reclaim 100g EL0024 Agarose Micro Plus 25g EL0025 Agarose Micro Plus 100g electrophoresis Blotting Protein electrophoresis such as radial immunodiffusion 12,000 - 500 0.8 10,000 - 500 8,000 - 300 1.0 7,000 - 250 6,000 - 200 1.2 5,000 - 200 3,500 - 100 1.5 3,000 - 100 2,000 - 50 2.0 2,000 - 50 Extra 40,000 - 3,000 0.3 20,000 - 2,000 Conventional electrophoresis Pulsed field gel electrophoresis Blotting Agarose bead preparation Cell and enzyme immobilisation 22,000 - 2,000 0.5 12,000 - 1,500 15,000 - 1,000 0.8 9,000 - 1,000 10,000 - 400 1.0 6,000 - 500 5,000 - 200 1.8 3,000 - 200 Reclaim 20,000 - 500 0.75 12,000 - 500 For applications that require efficient separation of small DNA fragments and PCR products 16,000 - 300 1.0 8,000 - 300 10,000 - 250 0.25 4,000 - 200 5,000 - 200 0.5 3,000 - 150 2,500 - 100 0.75 2,000 - 100 1,500 - 50 2.0 1,000 - 50 Micro Plus 1,500 - 500 2.0 1,200 - 400 Fragment separation in analytical and preparative electrophoresis Tissue and cell culture Viral plaque assays 1,200 - 300 3.0 800 - 100 600 - 150 4.0 500 - 50 250 - 30 5.0 250 - 20 Agarose Universal Agarose Extra Agarose Reclaim Agarose Micro Plus Moisture ≤ 7% < 7% ≤7% ≤ 7% Ash ≤4% < 0.25% ≤ 0.4% ≤ 0.35% EEO 0.05 - 0.13% < 0.12 ≤ 0.12 ≤ 0.12 Sulphate < 0.1% < 0.12% ≤ 0.12% ≤ 0.11% Clarity (NTU) ≤ 4 < 4 ≤ 4 ≤ 5 Gel Strength 1% (g/cm2) ≥ 1200 > 1800 - ≥ 4200 (4%) Gel Strength 1.5% (g/cm2) ≥ 2500 > 3200 ≥ 500 ≥ 2000 Gelling Temperature (C) 36 ± 1.5 36 + 1.5 24 - 28 ≤ 40.5 Melting Temperature (C) 88 ± 1.5 88 + 1.5 ≤ 65.5 ≤ 93 DNase/RNase activity None detected None detected None detected None detected DNA resolution Finely resolved Finely resolved Finely resolved Finely resolved Gel background Very low Very low Very low Very low


Catalogue_WEB_WoP_Jun16
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