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Research Reagents MicroRNA Isolation Kits MicroRNA Extractor SP Circulating microRNA in blood and other body fluids may be associated with disease and have been proposed as potential biomarkers for cancer and other diseases. The microRNA Extractor SP kit is designed for extraction of micro-RNA from serum or plasma without the use of phenol or chloroform. The microRNA fractions recovered are suitable for analysis by quantitative PCR. Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 212 Further investigation of miRNA can be aided by the microRNA cloning kit which allows synthesis of cDNA from miRNA. Alternatively target mRNA can be used as the starting material using the target mRNA cloning kit, by using RNA fractions from Ago immunoprecipitated protein the cDNA is generated from mRNA interacting with miRNA. MicroRNA and mRNA Cloning Catalogue Number Description Pack Size 290-66501 microRNA Cloning Kit wako 8 Tests 298-68001 Target mRNA Cloning Kit Wako 10 Tests MicroRNA Isolation Kits Catalogue Number Description Pack Size 291-70201 microRNA Isolation Kit, Human/Mouse Ago1 10 Tests 292-66701 microRNA Isolation Kit, Human Ago2 10 Tests 292-67301 microRNA Isolation Kit, Mouse Ago2 10 Tests 297-70301 microRNA Isolation Kit, Human Ago3 10 Tests MagCapture™ MicroRNA Isolation kits The MagCapture™ microRNA isolation kit series utilise antibodies immobilised on magnetic beads, enabling magnetic separation of RNA. Available in a choice of Human Ago2 (also reacts with monkey & dog), Mouse Ago2 (also reacts with rat & dog) or Ago1-4 (reacts with human, monkey, dog, mouse & rat) these kits can be used with cells and tissue samples but can also be used to recover mRNA from serum or plasma. Additionally a Protein G MagCapture microRNA Isolation Kit is offered enabling the user to immobilise an anti-ago antibody of choice for isolation of miRNA from cell and tissue samples. MagCapture™ MicroRNA Isolation kits Catalogue Number Description Pack Size 295-74001 MagCapture microRNA Iso Kit, Human Ago2 10 Tests 297-74201 MagCapture microRNA Iso Kit, Mouse Ago2 10 Tests 293-74801 MagCapture microRNA Iso Kit, Ago1-4 10 Tests 299-74401 MagCapture microRNA Iso Kit, Protein G 10 Tests MicroRNA Extractor SP Catalogue Number Description Pack Size 295-71701 MicroRNA Extractor SP Kit 50 Tests Certain Ago antibodies have been incorporated into dedicated microRNA isolation kits. The anti-Ago antibody is used to immunoprecipitate the Ago protein together with associated RISC complex and miRNA. RNA isolated with these kits can then be used in downstream applications such as quantitative PCR, cloning or microarray. The microRNA isolation kits use antibody conjugated silica-beads which enable centrifuge immunoprecipitation of both miRNAs and mRNA. Suitable for use with cells or tissues, following immunoprecipitation the RNA is purified by phenol/chloroform and ethanol precipitation steps. Four kit options to choose from: Human/Mouse Ago1, Human Ago2 (also reacts with monkey & dog), Mouse Ago2 (also reacts with rat) and Human Ago3. MicroRNA and mRNA Cloning Nucleic Acid Research


Catalogue_WEB_WoP_Jun16
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