Page 213

Catalogue_WEB_WoP_Jun16

Pricing and Online Ordering at www.alphalabs.co.uk Research Reagents Nucleic Acid Research Nucleic Acid Research Nucleic acids are one of the key building blocks of life. Nucleotides assembled into polymeric macromolecules like DNA and RNA serve to carry essential biological information for organism development and function. DNA encodes and stores the genetic master template from which RNA sequences are transcribed, and in the case of coding sequences these messenger RNAs (mRNAs) may be subsequently translated into proteins. Studying the interactions of these nucleic acids has revealed complex, multi-faceted pathways of gene regulation and interaction. Changes in gene expression may be associated with various diseases and elucidating the mechanisms behind such dysregulation could help shape future therapies. MicroRNA Research Tools Argonaute Protein Antibodies The Argonaute protein family is subdivided into Argonaute (Ago); PIWI (named after the drosophilia protein P-element-induced wimpy testis); and nematode-specific worm Argonaute (Wago) proteins. Ago proteins appear to be expressed everywhere whilst Piwi protein expression is restricted mainly to the germ line. Within the human genome there are four Ago proteins (Ago1-4) and four PIWI proteins (PIWIL1-4). Ago expression varies between cell types but the most abundantly expressed protein is Ago2, the only member of the Ago subfamily to exhibit slicer activity necessary for processing miRNA transcripts. Argonaute Protein Antibodies Catalogue Number Description Application Pack IP ICC RIP WB Size 015-22411 Anti-Ago1 MAb    50μl 018-22401 Anti-Ago1 MAb  50μl 011-22033 Anti-Ago2 (human) MAb     50μl 015-22031 Anti-Ago2 (human) MAb     100μl 014-22023 Anti-Ago2 (mouse) MAb     50μl 018-22021 Anti-Ago2 (mouse) MAb     100μl 018-23241 Anti-Ago3 (human) MAb   50μl 010-23821 Anti-Ago3 (human) MAb  50μl 016-25501 Anti-Ago3 (mouse) MAb   50μl 013-25491 Anti-Ago3 (mouse) MAb  50μl 019-24751 Anti-Ago4 MAb   50μl 012-24741 Anti-Ago4 MAb  50μl 012-25581 Anti-Ago1-4 MAb   200μl 018-25583 Anti-Ago1-4 MAb   1ml 016-25584 Anti-Ago1-4 MAb   5ml 017-23451 Anti-PIWIL1MAb   100μl 018-23981 Anti-PIWIL2 (human) MAb  50μl 015-23991 Anti-PIWIL2 (human) MAb  50μl MicroRNAs (miRNAs) are small non-coding RNA sequences, typically around 22 nucleotides in length, which act as post-transcriptional regulators of gene expression. Mature miRNAs are localised in the cytoplasm where they bind and assemble into the RNA-induced silencing complex (RISC) to target messenger RNA (mRNA). At the heart of every RISC is a member of the Argonaute protein family, which serves to bind the miRNAs and position them in a way that facilitates target recognition. The miRNA bind complementary sequences in the target and the RISC may then directly cleave the target RNA or recruit other gene-silencing proteins to inhibit translation. Wako offers a range of Ago antibody based products to study Ago protein expression and investigate the miRNA and mRNA that associate with Ago proteins in the RISC complex. n email order sales@alphalabs.co.uk n freefax order 0800 614249 211


Catalogue_WEB_WoP_Jun16
To see the actual publication please follow the link above