Research Reagents In vitro culture is a cornerstone of numerous disciplines with a diversity of applications including toxicity and drug screening, cell signalling and pathway study, developmental biology and tissue regeneration with stem cells. Successful cultivation is dependent on providing a suitable environment for the cell type in question. 208 Cell and Tissue Culture Products Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk Cell and Tissue Culture Products The PrimeSurface range of cultureware was developed by the S-Bio division of the Sumitomo Bakelite Company to provide plastic culture vessels with low cell adhesion properties. SUMILON’s proprietary low-protein-adsorption technology prevents cell adhesion making these ideal for spheroid cell colony formation. PrimeSurface products provide stable culture surfaces with a ultra-hydrophilic polymer coating PrimeSurface Cultureware Catalogue Number PrimeSurface Cultureware containing a ‘high density –OH group’ which prevents elution of chemicals from the plate which can influence cell response and also promotes spontaneous spheroid formation within stem cell cultures. Following EB formation the ES cells can be stimulated to induce cell differentiation. PrimeSurface culture plates have been successfully used to induce differentiation of ES, iPS and nerve cells. Description Colour Well Bottom Volume Pack Size MS-9024X PrimeSurface 24 Well Plate Clear Culture area 1.8cm2 3.4ml 10 plates MS-9096U PrimeSurface 96 Well U Bottom Plate Clear 300μl 20 plates MS-9096W PrimeSurface 96 Well U Bottom Plate White 300μl 20 plates MS-9096M PrimeSurface 96 Well Spindle Bottom Plate Clear 200μl 20 plates MS-9096V PrimeSurface 96 Well V Bottom Plate Clear 300μl 20 plates MS-9035X PrimeSurface dish 35mm Clear Culture area 9cm2 - 5 x 10 dishes Celltight Cultureware Celltight Cultureware - Collagen Catalogue Number Description Pack Size MS-0035K Celltight C-1 Dish 35mm 100 MS-0060K Celltight C-1 Dish 60mm 100 MS-0090K Celltight C-1 Dish 90mm (S) 40 MS-0390K Celltight C-1 Dish 90mm (D) 50 MS-0006K Celltight C-1 Plate 6F 20 MS-0012K Celltight C-1 Plate 12F 20 MS-0024K Celltight C-1 Plate 24F 20 MS-0048K Celltight C-1 Plate 48F 20 MS-0096K Celltight C-1 Plate 96F 20 MS-0405K Celltight C-1 Flask 50ml 50 MS-0425K Celltight C-1 Flask 250ml 20 MS-0480K Celltight C-1 Flask 800ml 10 MS-0096WK Celltight C-1 Plate 96F White 20 MS-0096KK Celltight C-1 Plate 96F Black 20 Whilst some cells lines readily adhere to a plastic culture surface other cell types may be a little more demanding and require the additional support of an extracellular matrix protein. The Celltight range of culture ware has been pre-coated with collagen, gelatin or Poly-L-Lysine to enhance cell adhesion and is well suited to use with primaries and nerve cells.
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