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Catalogue_WEB_WoP_Jun16

Research Reagents Automated Protein Sequencing The Edman degradation method for amino acid sequencing of proteins and peptides was described by Edman in 1950 and later developed into an automated method by Edman and Begg. Wako produces a range of chemicals and consumables recommended by Shimadzu for use with their automated Protein Primary Structure Analysers (PPSQ-1, PPSQ-2 and PPSQ-10). Following SDS-PAGE protein expression may be analysed by staining with Ponceau, Coomassie Brilliant Blue, or Negative Gel Stain as a precursor to sequencing with mass-spectrophotometry. Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 202 Automated Protein Sequencing Catalogue Number Description Pack Size 014-12471 Acetonitrile 200mL 014-13831 37% Acetonitrile Solution  200mL 021-09851 1-Chlorobutane 200mL 054-04981 Ethyl Acetate  200mL 071-03131 Glass Fiber Disk, TFA Treated (12mm diameter) 50 Sheets 073-04171 Glass Fiber Disk, TFA Treated (8mm in diameter) 50 Sheets 084-05501 Heptane 200mL 168-14161 5% Phenyl Isothiocyanate n-Heptane Solution 40mL 204-10771 Trifluoroacetic Acid 40mL 201-10781 25% Trifluoroacetic Acid Solution (glass container) 40mL 203-12201 25% Trifluoroacetic Acid Solution (plastic container) 40mL 207-10761 12% Trimethylamine Solution 200mL 161-14271 PTH-Amino Acids Mobile Phase 1 Litre 239-63351 Wakopak® Wakosil-PTH, 4.6 mm × 250 mm 1 Column Gel Stains Gel Stains Catalogue Number Description Pack Size 169-18915 Ponceau-3R Stain 500ml 299-50101 Quick CBB 2L 174-00553 Quick CBB Plus 250ml 178-00551 Quick CBB Plus 1L 293-57701 Negative Gel Stain MS Kit 20 Tests Mass Spectrometry Protein Analysis Following electrophoresis, distinct protein bands or spots of interest can be subjected to in-gel digestion by enzymes such as lysyl endopeptidase or endoproteinases prior to analysis by MALDI-TOF MS. Wako offers a range of products to optimise such analysis from proteome preparation reagents and digestion enzymes to MALDI-TOF MS matrix. Mass Spectrometry Protein Analysis Catalogue Number Description Pack Size 164-22691 Proteome Preparation Reagent 100μl 087-08891 Hydrophobic Protein Preparation Reagent 200μl 037-19261 α-Cyano-4-hydoxycinnamic Acid (CHCA) 5 x 50mg 192-13361 Sinapic Acid (SA) 5 x 50mg 044-29101 2,5-Dihydroxybenzoic Acid (DHB) 5 x 50mg 048-29481 2,5-Dihydroxybenzoic Acid Lithium Salt 50mg 041-29471 2,5-Dihydroxybenzoic Acid Sodium Salt 50mg 125-05061 Lysyl Endopeptidase MS Grade 5 x 20μg 202-15951 Trypsin MS Grade 5 x 20μg 056-05921 Endoproteinase Asp N 2μg 050-05941 Endoproteinase Glu C 50μg 164-13982 V8 Protease 2mg Proteomics


Catalogue_WEB_WoP_Jun16
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