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Pricing and Online Ordering at www.alphalabs.co.uk Research Reagents Proteomics Protein Electrophoresis Reagents Proteins run on native, or non-denaturing PAGE will separate on the basis of their charge to mass ratio. Whilst this method does not provide direct measurement of molecular weight, as progress through the gel will be influenced by the protein structural conformation, proteins on native gels will usually retain their activity making this technique well suited to the study of enzymes to be detected by activity stains or recovered in a biologically active form. More commonly samples are denatured, typically with sodium dodecyl sulphate (SDS) which gives the protein a uniform charge. This charge/mass ratio is consistent between proteins meaning that separation on SDS-PAGE is dependent upon protein mass enabling molecular weight measurement. Wako offers a range of laemmli sample buffers and running buffers for PAGE. Also offered are WIDE-VIEW III protein markers. Pre-stained, these markers have a range of 11- 145kDa with coloured proteins demarking 75kDa (red) and 25kDa (green). Protein Electrophoresis Reagents Catalogue Number Description Pack Size 193-11032 Sample Buffer Solution (-2ME) (X2) 25ml 198-13282 Sample Buffer Solution (-2ME) (X4) 25ml 199-16132 Sample Buffer Solution (TG) (X2) 25ml 196-16142 Sample Buffer Solution TG) (X4) 25ml 184-01291 Running Buffer Solution (X10) 1L 200-17071 Tricine Running Buffer 1L 201-18601 Tris-Glycine Buffer 1L 230-02461 WIDE-VIEW Pre-stained Protein Marker 500μL SDS Solution Alpha Laboratories’ SDS solution (20%) is formulated using the highest quality SDS, that has been further purified to remove contaminants, which can interfere with spectro-photometric analysis and cause non-specific binding. The purity of this product ensures that reproducible protein molecular weight measurements can be made with confidence, and it optimises activity recovery in enzyme purification procedures. The convenient pre-prepared solution eliminates the discomfort and hazards of working with powdered SDS. SDS Solution Catalogue Number Description Pack size EL0094 SDS Solution 20% (w/v) 100ml SDS - ULTRA PURE (Sodium Dodecyl Sulphate) Used as a powerful protein denaturant and solubilising agent, which induces conformational changes in proteins at low concentrations, enabling proteins to be separated on the basis of their molecular weight. The purity of these products ensures that reproducible protein molecular weight measurements can be made with confidence. Purified to remove coloured contaminants that may interfere with spectrophotometric analysis. Zero free sulphates eliminate non-specific binding. Specifications: Molecular Weight 288.38 Purity ≥99% OD280 (3% Solution) < 0.1 C12 Sulphate ≥99% Lead (Pb) < 0.0002% Phosphate (PO4) < 0.0001% SDS – ULTRA PURE (Sodium-Dodecyl Sulphate) Catalogue Number Description Pack size EL0070 Sodium Dodecyl Sulphate 1kg n email order sales@alphalabs.co.uk n freefax order 0800 614249 201


Catalogue_WEB_WoP_Jun16
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