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Research Reagents Proteomics The wide-scale analysis of gene products has been a growing area of interest as mRNA analysis is not always concordant with protein expression. Furthermore, post-transcriptional modifications are of key importance as these occur in response to a variety of intracellular and extracellular signals. Alpha offer a range of products to aid proteomic investigations from separation to sequencing along with the Phos-tag™ series specifically designed for analysis of protein phosphorylation. Phos-tag™ Biotin Phos-tag™ biotin ligands can be used to detect phosphorylated proteins on PVDF membrane as an alternative to anti-phosphorylated antibodies. Unlike traditional immunodetection the membranes can be probed without blocking and phos-tag biotin is sensitive enough to detect phosphorylated protein at nanogram levels. Subsequently membranes can easily be stripped and re-probed Pricing and Online Ordering at www.alphalabs.co.uk n freephone order 0800 387732 n buy online at www.alphalabs.co.uk 200 Phos-tag™ Labelling The Phos-tag™ developed by Hiroshima University is a functional molecule that binds phosphorylated ions, forming stable complexes under physiological conditions. This has been used to develop a range of products to assist the study of phosphorylation. Phos-tag acrylamide can be used for mobility shift detection of phosphorylated proteins using phosphate affinity SDS-PAGE. Phos-tag labelled acrylamide is incorporated into the gel and subsequently during electrophoresis serves to retard the progress of phosphorylated proteins through the gel enabling separation of phosphorylated and non-phosphorylated isomers. Phos-tag™ Acrylamide Catalogue Number Description Pack Size 304-93526 Phos-tag Acrylamide AAL-107, 5mM 300μl 300-93523 Phos-tag Acrylamide AAL-107 2mg 304-93521 Phos-tag Acrylamide AAL-107 10mg Proteomics with conventional antibodies if desired. Phos-tag™ Biotin Catalogue Number Description Pack Size 301-93531 Phos-tag Biotin BTL-104 10mg 308-93541 Phos-tag Biotin BTL-105 10mg 308-97201 Phos-tag Biotin BTL-111 1mM 0.1ml Phos-tag™ Agarose Phos-tag™ agarose can be used to construct a phosphate affinity column enabling purification of phosphorylated proteins suitable for down-stream analysis such as mass spectrometry or SDS-PAGE. Phos-tag™ Agarose Catalogue Number Description Pack Size 302-93561 Phos-tag Agarose 500μl 308-93563 Phos-tag Agarose 3mL Mass Spectrometry Kit The Phos-tag™ mass analytical kit can be used to provide rapid and sensitive matrix-assisted laser desorption/ ionisation-time of flight mass spectrometry using natural zinc and stable isotopes 64Zn and 68Zn. Mass Spectrometry Kit Catalogue Number Description Pack Size 305-93551 Phos-tag Mass Analytical Kit 1 set N RDinstitute,ltd. Phos-tag™ Acrylamide


Catalogue_WEB_WoP_Jun16
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