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Catalogue_WEB_WoP_Jun16

Laboratory Equipment - PCR Bioer LineGene™ Real-Time Thermal Cycler The Basic Principles of Real-Time PCR Real-time PCR, sometimes known as qPCR enables quantification of nucleic acids in real time with high sensitivity. During the polymerase chain reaction a fluorescent dye is incorporated into the DNA as it is synthesised. The instrument scans the samples during every cycle, providing accurate quantification of the PCR product as it multiplies. This negates the need for downstream processes such as electrophoresis and can provide very rapid results. The system incorporates melt curve analysis which enables post amplification investigation of the PCR products, each of which will have its own unique melting curve. This facilitates identification of the DNA fragments and differentiation of specific and non-specific products. uk co.alphalabs.www.at Ordering Online Key Benefits LineGene 9600 Real-Time Thermal Cyclers Performance and The LineGene 9600 series of instruments are 96 well ■■Ramping rate 4C/second Real-Time PCR systems capable of performing Absolute and Pricing Relative Quantification together with SNP and melt curve ■■Detection range 10 – 1010 copies analysis. ■■Real-time fast double wavelength X-Y scan system The LineGene 9600 is compatible with SBS footprint PCR ■■High sensitivity PMT (photomultiplier tube) x 2 plates, 8- or 12- strip-tubes and single 0.2ml tubes. This ■■Patented block dissipation technology provides greater flexibility whether you are performing high throughput or low throughput applications. Being more ■■Unique Bottom detection method with specially versatile than high throughput specific systems, the LineGene designed Peltiers 9600 series are ideally suited to meet the needs of a modern Flexibility research laboratory. ■■Capacity: 96 well plate format, 8- or 12- strip tubes and The LineGene 9600 systems are equipped with gradient 0.2ml tubes functionality for rapid optimisation, and have a melting temperature calculator – useful for establishing protocols. ■■Reaction volumes 10-100μl There is a choice of block or tube temperature regulation. ■■Open platform to allow use with different chemistries The latter uses an internal algorithm which calculates the ■■Up to 8 Wavelength Optical filters (2 can be customised) Cycler temperature of the actual sample in real time during the PCR ■■Instrument capability – process. --SNP Genotyping Thermal The hot-lid temperature can be set as required and the --Absolute Quantification height adjusts automatically to the size of the tube or plate, effectively preventing evaporation. --Relative Quantification Time --Melt Curve Analysis --Gradient function Real LineGene Real-Time Thermal Cycler --Multiplexing Catalogue Description ™Number Ease and Reliability LineGeneTC5020 LineGene9620, 2 channel qPCR Machine TC5010 LineGene9640, 4 channel qPCR Machine ■■Easy-to-use software TC5000 LineGene9660, 6 channel qPCR Machine TC5030 LineGene9620, 8 channel qPCR Machine ■■High light long life LED 152n freephone order 0800 387732 n buy online at www.alphalabs.co.uk


Catalogue_WEB_WoP_Jun16
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